• In vivo Imaging

• Whole-mount Fluorescent in vivo imaging
• BigDye Sequencing
• Zebrafish spinal canal antibody staining
  



• CRISPR synthesis
  

• DIG antisense probe
  

• Insitu hybridization protocol
  

• Fluorescent In Situ
  

• Homemade Gibson Cloning
  

• Lentivirus production
  

• Carpetbombing with CRISPR (Johnson Lab)
  

Mouse Protocols

• Skeletal prep protocol
  

• Isolation of primary mouse chondrocytes
  

• Tamoxifen injection Mouse
  

• Mouse Hot Shot DNA extraction for genotyping
  

The Zebrafish Book

• Egg Water
• Triciane
• Methylene Blue
• Rotifer culture
• Harvesting Rotifers