The Gray Lab at the Dell Pediatrics Research Institute
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Genotyping Protocols

Mouse

ALLELE
NOTES
NOTES
Kif6em1Rgray
C57BL/6J-Kif6em1(R556Vfs*7)Rgray/Mmnc
Kif6-C terminal truncation
Kif6-LacZtm1b
B6(Cg)-Kif6tm1b(KOMP)Mbp/RgrayMmnc
Kif6-LacZ reporter
Kif6tm1c
B6J;129S4-Kif6tm1c(KOMP)Mbp/RgrayMmnc
Kif6 floxed allele

Zebrafish

ALLELE
NOTES
scospondin-hsc105
strong 'null' allele of scospondin, curly tail down phenotype

Zebrafish Transgenics/Knock-in Lines

ALLELE
Received from/Made by
External Reference
Screening Image
Tg(foxj1a:ARL13B-GFP)-ut22
Ciruna Lab TOL2 plasmid/Ryoko Minowa/Ben Troutwine
https://zfin.org/ZDB-ALT-190731-4

scospondin-GFP-ut24-knock-in
Ben Troutwine
Hard to detect on a 1X epifluorescent scope; higher mag can see it in the spinal cord.

Zebrafish

  • kif6-sko
  • kif6-P293T
  • kurly-tm304
  • inppl1a-stl445

Mouse

Improved mouse CRE
Sox9-Asp272del
Sox9-flox

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  • Reagents and Lab Docs
  • Genotyping protocols